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Anticorps Recombinant de lapin anti-SOX2

SOX2 Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230225G1

N° de cat : 83059-5-PBS

Synonymes

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SOX2 Recombinant antibody (83059-5-RR)

    IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).CL594-Phalloidin (red). This data was developed using the same antibody clone with 83059-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SOX2 Recombinant antibody (83059-5-RR)

    IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83059-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83059-5-RR

    Affinity and Kinetic Characterization of 83059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83059-5-RR against Human SOX2 were performed. The affinity constant is 3.54 nM.

Informations sur le produit

83059-5-PBS cible SOX2 dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène SOX2 Protéine recombinante Ag0612
Nom complet SRY (sex determining region Y)-box 2
Masse moléculaire calculée 34 kDa
Numéro d’acquisition GenBankBC013923
Symbole du gène SOX2
Identification du gène (NCBI) 6657
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undiferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal diferentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ) , a well-known stem cell niche of the adult brain.

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