Anticorps Monoclonal anti-SOX2

• Phare
• Validé par KD/KO

SOX2 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4B9B10

N° de cat : CL594-66411

Synonymes

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

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Galerie de données de validation

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  IF Staining of mouse brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66411 (SOX2 antibody) at dilution of 1:50.

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  IF Staining of rat brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:400.

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  IF Staining of mouse brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200. DAPI (blue).

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  IF Staining of U-251 using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200.

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  FC experiment of U-251 using CL594-66411

  1X10^6 U-251 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SOX2 (CL594-66411, Clone:4B9B10) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules U-251,
• Résultats positifs en FC (Intra): cellules U-251,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66411 cible SOX2 dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SOX2 Protéine recombinante Ag13635
• Nom complet: SRY (sex determining region Y)-box 2
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC013923
• Symbole du gène: SOX2
• Identification du gène (NCBI): 6657
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

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