Anticorps Recombinant de lapin anti-SOX4

• Phare
• Validé par KD/KO

SOX4 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240625E3

N° de cat : 83660-1-RR

Synonymes

240625E3, CSS10, EVI16, Transcription factor SOX-4

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Galerie de données de validation

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  WB analysis of MCF-7 using 83660-1-RR

  WB result of SOX4 antibody (83660-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX4 transfected MCF-7 cells.

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  WB analysis using 83660-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 83660-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of HeLa using 83660-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83660-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules MCF-7, cellules SGC-7901
• Résultats positifs en FC (Intra): cellules SH-SY5Y, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83660-1-RR cible SOX4 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SOX4 Protéine recombinante Ag26583
• Nom complet: SRY (sex determining region Y)-box 4
• Masse moléculaire calculée: 474 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC072668
• Symbole du gène: SOX4
• Identification du gène (NCBI): 6659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa.

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