- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-SOX9
SOX9 Monoclonal Antibody for WB, FC (Intra), ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, souris et plus (4)
Applications
WB, FC (Intra), ELISA
Conjugaison
Non conjugué
CloneNo.
1H9A6
N° de cat : 67439-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HT-29, cellules Caco-2, cellules NCCIT, tissu placentaire humain |
Résultats positifs en IHC | tissu de gliome humain, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en FC (Intra) | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochimie (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 1 publications below |
WB | See 67 publications below |
Informations sur le produit
67439-1-Ig cible SOX9 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, canin, Humain, Lapin, porc, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | SOX9 Protéine recombinante Ag28620 |
Nom complet | SRY (sex determining region Y)-box 9 |
Masse moléculaire calculée | 509 aa, 56 kDa |
Poids moléculaire observé | 56 kDa |
Numéro d’acquisition GenBank | BC007951 |
Symbole du gène | SOX9 |
Identification du gène (NCBI) | 6662 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.
Protocole
Product Specific Protocols | |
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WB protocol for SOX9 antibody 67439-1-Ig | Download protocol |
IHC protocol for SOX9 antibody 67439-1-Ig | Download protocol |
FC protocol for SOX9 antibody 67439-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Bioact Mater Synergizing adaptive immunity and regenerative signals to enhance osteochondral defects repair | ||
Sci Transl Med Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology | ||
Adv Sci (Weinh) Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis | ||
Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI | ||
Mater Today Bio Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction | ||
Stem Cell Res Ther Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Iram (Verified Customer) (09-18-2025) | Very good antibody for IB, IHC and IF.
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FH Agustina (Verified Customer) (09-13-2025) | With this dilution the cells included as positive control (PC3 cells) had a good signal, however not much in the PDXs, even though it seems some was there (could be related with decalcification or other sample processing steps). I will try increasing the antibody concentration.
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