• Phare
  • Validé par KD/KO

Anticorps Monoclonal anti-SOX9

SOX9 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (4)

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(87)

CloneNo.

1H9A6

N° de cat : 67439-1-Ig

Synonymes

1H9A6, CMD1, CMPD1, SRA1, Transcription factor SOX 9



Applications testées

Résultats positifs en WBcellules HT-29, cellules Caco-2, cellules NCCIT, tissu placentaire humain
Résultats positifs en IHCtissu de gliome humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en FC (Intra)cellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

67439-1-Ig cible SOX9 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, canin, Humain, Lapin, porc, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène SOX9 Protéine recombinante Ag28620
Nom complet SRY (sex determining region Y)-box 9
Masse moléculaire calculée 509 aa, 56 kDa
Poids moléculaire observé 56 kDa
Numéro d’acquisition GenBankBC007951
Symbole du gène SOX9
Identification du gène (NCBI) 6662
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.

Protocole

Product Specific Protocols
WB protocol for SOX9 antibody 67439-1-IgDownload protocol
IHC protocol for SOX9 antibody 67439-1-IgDownload protocol
FC protocol for SOX9 antibody 67439-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat,mouse

Bioact Mater

Synergizing adaptive immunity and regenerative signals to enhance osteochondral defects repair

Authors - Changjian Lin
humanWB

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
rat,humanWB

Adv Sci (Weinh)

Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis

Authors - Yizhen Huang
mouse,humanWB

Theranostics

Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Authors - Bojun Li
humanWB

Mater Today Bio

Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

Authors - Zhanqiu Dai
mouseWB

Stem Cell Res Ther

Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia

Authors - Yonghao Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Iram (Verified Customer) (09-18-2025)

Very good antibody for IB, IHC and IF.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 for IB, 1:250 for IHC and 1:200for IF
  • Cell Tissue Type: CRC cells and tissues
FH

Agustina (Verified Customer) (09-13-2025)

With this dilution the cells included as positive control (PC3 cells) had a good signal, however not much in the PDXs, even though it seems some was there (could be related with decalcification or other sample processing steps). I will try increasing the antibody concentration.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human PDXs growing in mice femur, PC3 cell line pellet and PC3 subcutaneous tumor.
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