Anticorps Monoclonal anti-SOX9

• Phare
• Validé par KD/KO

SOX9 Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

41

CloneNo.

1H9A6

N° de cat : 67439-1-Ig

Synonymes

CMD1, CMPD1, SOX9, SRA1, Transcription factor SOX 9

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Galerie de données de validation

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  WB analysis using 67439-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human placenta using 67439-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 67439-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67439-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 67439-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67439-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 67439-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SOX9 (67439-1-Ig, Clone:1H9A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules Caco-2, cellules NCCIT, tissu placentaire humain
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 33 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Informations sur le produit

67439-1-Ig cible SOX9 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, Lapin, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SOX9 Protéine recombinante Ag28620
• Nom complet: SRY (sex determining region Y)-box 9
• Masse moléculaire calculée: 509 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC007951
• Symbole du gène: SOX9
• Identification du gène (NCBI): 6662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology; Authors - Su'an Tang
human,mouse	WB,IF	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
human	WB	Mater Today Bio; Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction; Authors - Zhanqiu Dai
rat	WB	J Nanobiotechnology; Dual-dynamic-bond cross-linked injectable hydrogel of multifunction for intervertebral disc degeneration therapy; Authors - Linjun Yang
mouse	WB	Free Radic Biol Med; Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes; Authors - Wenchao Wang
human	WB	EBioMedicine; Decreased miR-214-3p activates NF-κB pathway and aggravates osteoarthritis progression.; Authors - Yumei Cao