Anticorps Monoclonal anti-SOX9

• Phare
• Validé par KD/KO

SOX9 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1H9A6

N° de cat : CL647-67439

Synonymes

1H9A6, CMD1, CMPD1, SRA1, Transcription factor SOX 9

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Galerie de données de validation

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  FC experiment of HeLa using CL647-67439

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 647 Sox9 Monoclonal Antibody (CL647-67439, Clone:1H9A6)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (1F8D3) (CL647-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-67439 cible SOX9 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SOX9 Protéine recombinante Ag28620
• Nom complet: SRY (sex determining region Y)-box 9
• Masse moléculaire calculée: 509 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC007951
• Symbole du gène: SOX9
• Identification du gène (NCBI): 6662
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.

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