Anticorps Recombinant de lapin anti-SP100

SP100 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241153F3

N° de cat : 84012-4-PBS

Synonymes

241153F3, Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP 100, SP100 nuclear antigen

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Galerie de données de validation

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  WB analysis using 84012-4-RR (same clone as 84012-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84012-4-RR (SP100 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84012-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84012-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84012-4-RR against Human SP100 were performed. The affinity constant is 0.127 nM.

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Informations sur le produit

84012-4-PBS cible SP100 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SP100 Protéine recombinante Ag35193
• Nom complet: SP100 nuclear antigen
• Masse moléculaire calculée: 879 aa, 100 kDa
• Poids moléculaire observé: 70-100 kDa
• Numéro d’acquisition GenBank: BC011562
• Symbole du gène: SP100
• Identification du gène (NCBI): 6672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.

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