Anticorps Polyclonal de lapin anti-SP110

SP110 Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11502-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 11502-1-AP (same clone as 11502-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

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  WB analysis using 11502-1-AP (same clone as 11502-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11502-1-AP (same clone as 11502-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 11502-1-AP (same clone as 11502-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 11502-1-AP (same clone as 11502-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 11502-1-AP (same clone as 11502-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 11502-1-AP (same clone as 11502-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11502-1-AP (same clone as 11502-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 11502-1-AP (same clone as 11502-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 11502-1-AP (same clone as 11502-1-PBS)

  IP result of anti-SP110 (IP:11502-1-AP, 4ug; Detection:11502-1-AP 1:800) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 11502-1-AP (same clone as 11502-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SP110 (11502-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11502-1-PBS cible SP110 dans les applications de WB, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SP110 Protéine recombinante Ag2042
• Nom complet: SP110 nuclear body protein
• Masse moléculaire calculée: 689 aa, 79 kDa
• Poids moléculaire observé: 62-66 kDa
• Numéro d’acquisition GenBank: BC019059
• Symbole du gène: SP110
• Identification du gène (NCBI): 3431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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