Anticorps Recombinant de lapin anti-SP3

SP3 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242556A2

N° de cat : 85050-1-RR

Synonymes

242556A2, SPR 2, SPR-2

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Galerie de données de validation

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  WB analysis using 85050-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85050-1-RR (SP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 85050-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85050-1-RR (SP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 85050-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SP3 antibody (85050-1-RR, Clone: 242556A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85050-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85050-1-RR against Human SP3 were performed. The affinity constant is 2.38 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules C6, cellules COLO 320, cellules HCT 116, cellules K-562, cellules PC-3
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85050-1-RR cible SP3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SP3 Protéine recombinante Ag25082
• Nom complet: Sp3 transcription factor
• Masse moléculaire calculée: 781 aa, 82 kDa
• Poids moléculaire observé: 70 kDa, 115 kDa
• Numéro d’acquisition GenBank: BC126414
• Symbole du gène: SP3
• Identification du gène (NCBI): 6670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SP3, also named as transcription factor Sp3, is a 781 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. SP3 localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies. SP3 is acetylated by histone acetyltransferase p300, deacetylated by HDACs and sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. There exist tow modified isoform and molecular weight of those are 70 kda and 115 kda.

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