Anticorps Recombinant de lapin anti-SP7

SP7 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250286C5

N° de cat : 85934-1-PBS

Synonymes

osterix, OSX, Sp7 transcription factor, Transcription factor Sp7, Zinc finger protein osterix

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Galerie de données de validation

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  WB analysis using 85934-1-RR (same clone as 85934-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85934-1-RR (SP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85934-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85934-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85934-1-RR against Human SP7 were performed. The affinity constant is 1.10 nM.

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Informations sur le produit

85934-1-PBS cible SP7 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SP7 Protéine recombinante Ag29889
• Nom complet: Sp7 transcription factor
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_152860
• Symbole du gène: SP7
• Identification du gène (NCBI): 121340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sp7 is identified in several tissues, including bone, tooth, brain, and the reproductive tract (PMID: 11792318, 33135214, 36881265). In bone, Sp7 is expressed at multiple stages throughout the mesenchymal bone lineage and plays different roles in different osteocytes (PMID: 36881265). SP7 regulates osteoblast differentiation and bone formation through osteoblast target genes by recognizing AT-rich region. In osteocytes, SP7 regulates osteocyte maturation and intracortical remodeling through osteocyte target genes by recognizing TGA(G/T) TCA motif. SP7 also regulates chondrocyte differentiation and endochondral ossification (36881265).

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