Anticorps Polyclonal de lapin anti-SPA17

SPA17 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13367-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13367-1-AP (SPA17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

  IP result of anti-SPA17 (IP:13367-1-AP, 3ug; Detection:13367-1-AP 1:400) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13367-1-PBS cible SPA17 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPA17 Protéine recombinante Ag4186
• Nom complet: sperm autoantigenic protein 17
• Masse moléculaire calculée: 151 aa, 17 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC032457
• Symbole du gène: SPA17
• Identification du gène (NCBI): 53340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.

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