Anticorps Polyclonal de lapin anti-SPAG16

SPAG16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16883-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of rat testis using 16883-1-AP (same clone as 16883-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 16883-1-AP (same clone as 16883-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 16883-1-AP (same clone as 16883-1-PBS)

  IP result of anti-SPAG16 (IP:16883-1-AP, 4ug; Detection:16883-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16883-1-AP (same clone as 16883-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using SPAG16 antibody 16883-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16883-1-PBS cible SPAG16 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPAG16 Protéine recombinante Ag10348
• Nom complet: sperm associated antigen 16
• Masse moléculaire calculée: 631 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC009614
• Symbole du gène: SPAG16
• Identification du gène (NCBI): 79582
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPAG16 (also known as PF20) is an orthologue of Chlamydomonas PF20, a component of the alga axoneme central apparatus that is required for flagellar motility. SPAG16 is highly expressed in the testis, low abundant or absent from other tissues. The SPAG16 gene encodes two major proteins, a 71-kDa SPAG16L and a smaller 35-kDa SPAG16S nuclear protein that represents the C-terminus of SPAG16L. SPAG16L was detected abundantly in the cytoplasm, while SPAG16S was detected in both cytoplasm and nucleus. This antibody was raised against the N-terminal region of SPAG16 and mainly recognizes SPAG16L.

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