Anticorps Polyclonal de lapin anti-SPAG4-Specific

SPAG4-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19721-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPAG4-Specific antibody (19721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19721-1-PBS cible SPAG4-Specific dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sperm associated antigen 4
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: NM_003116
• Symbole du gène: SPAG4
• Identification du gène (NCBI): 6676
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4. We observed that the molecular weight of SPAG4-Specific was 55 kDa.

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