Anticorps Polyclonal de lapin anti-SPAG8

SPAG8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13915-1-PBS

Synonymes

HSD-1, HSD 1, CT142, CILD28, BS84

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Galerie de données de validation

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  WB analysis using 13915-1-AP (same clone as 13915-1-PBS)

  DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 13915-1-AP (SPAG8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13915-1-AP (same clone as 13915-1-PBS)

  IP result of anti-SPAG8 (IP:13915-1-AP, 3ug; Detection:13915-1-AP 1:500) with mouse testis tissue lysate 6000ug. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 13915-1-AP (same clone as 13915-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SPAG8 antibody (13915-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13915-1-PBS cible SPAG8 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPAG8 Protéine recombinante Ag4910
• Nom complet: sperm associated antigen 8
• Masse moléculaire calculée: 45 kDa, 54 kDa
• Poids moléculaire observé: 56-70 kDa
• Numéro d’acquisition GenBank: BC019247
• Symbole du gène: SPAG8
• Identification du gène (NCBI): 26206
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPAG8, also named as Sperm-associated antigen 8 or HSD-1, is a 426 amino acid protein, which is expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. SPAG8 may play a role in fertility and microtubule formation through interaction with RANBP9. SPAG8 as testis-specific protein is produced during male germ cell differentiation and functions in a germ cell type- and stage-specific manner during spermatogenesis.

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