Anticorps Polyclonal de lapin anti-SPAM1

SPAM1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 11208-1-AP

Synonymes

HYA1, Hyal PH20, HYAL1, HYAL3, HYAL5, Hyaluronoglucosaminidase PH 20, PH 20, PH20, SPAG15, SPAM1, Sperm adhesion molecule 1, Sperm surface protein PH 20

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Galerie de données de validation

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  WB analysis of PC-3 using 11208-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11208-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11208-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules MCF-7, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

11208-1-AP cible SPAM1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPAM1 Protéine recombinante Ag1682
• Nom complet: SPAM1
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: BC026163
• Symbole du gène: SPAM1
• Identification du gène (NCBI): 6677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.

Publications

Species	Application	Title
bovine	WB	Int J Biol Macromol; TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.; Authors - Na Wang

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