Anticorps Polyclonal de lapin anti-SPANXA2/SPANXA1

SPANXA2/SPANXA1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 19527-1-AP

Synonymes

Cancer/testis antigen 11.1, CT11.1, SPAN X, SPANX, SPANX A, SPANX family member A, SPANX family, member A2, SPANXA, SPANXA1, SPANXA2

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Galerie de données de validation

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  WB analysis of DU 145 using 19527-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19527-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19527-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19527-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

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  IHC staining of human testis using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

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  IHC staining of human skin cancer using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

• 

  IHC staining of human testis using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

• 

  IF Staining of HEK-293 using 19527-1-AP

  Immunofluorescent analysis of HEK-293 cells using 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19527-1-AP cible SPANXA2/SPANXA1 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SPANX family, member A2
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: NM_145662
• Symbole du gène: SPANXA2
• Identification du gène (NCBI): 728712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPANXA1, also named as SPANXA and SPANXA2, is a member of the SPANX family of cancer/testis-associated genes. It encodes differentially expressed testis-specific proteins that localize to various subcellular compartments. On Western blots of human testis proteins, SPANX antibodies reacts with the bands migrating between 15-30 kDa. The SPANX protein may be a useful target in cancer immunotherapy.