Anticorps Polyclonal de lapin anti-SPARC

• Phare
• Validé par KD/KO

SPARC Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15274-1-PBS

Synonymes

Osteonectin, ON, BM-40, BM 40, Basement-membrane protein 40

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Galerie de données de validation

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  WB analysis of ROS1728 using 15274-1-AP (same clone as 15274-1-PBS)

  A375 cells, ROS1728 cells, and human placenta tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 15274-1-AP (same clone as 15274-1-PBS)

  WB result of SPARC antibody (15274-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPARC transfected A375 cells. This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

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  WB analysis using 15274-1-AP (same clone as 15274-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 15274-1-AP (same clone as 15274-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15274-1-PBS cible SPARC dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPARC Protéine recombinante Ag7390
• Nom complet: secreted protein, acidic, cysteine-rich (osteonectin)
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-43 kDa
• Numéro d’acquisition GenBank: BC004974
• Symbole du gène: SPARC
• Identification du gène (NCBI): 6678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Secreted Protein Acidic And Cysteine Rich (SPARC), also known as osteonectin or basement-membrane protein 40 (BM-40), is a secreted protein essential in the calcification of bone. 

What is the molecular weight of SPARC?

The calculated molecular mass of SPARC is 35 kDa. SPARC is a glycoprotein consisting of 303 amino acids in the cytoplasm and 286 amino acids after the signal sequence is cleaved in secretion. 

Where is SPARC expressed?

Osteoblasts secrete SPARC during bone formation, with high levels found in immature bone compared to mature bone that is in homeostasis (PMID: 2440898). SPARC is also secreted in adult mineralized tissues that have high turnover, such as osteoid and dentin, by cells other than osteoblasts. This includes bone marrow progenitor cells and hypertrophic chondrocytes but also endothelial cells and fibroblasts.

Though previously described as being exclusively expressed in mineralized tissue, it is now understood that SPARC is more widely expressed and is associated with high levels of collagen deposition, found in the extracellular matrix (ECM) of a number of tissues.

What is the function of SPARC?

SPARC contains both a collagen-binding domain and a hydroxyapatite (HA) binding region, so is thought to enhance mineralization by binding both collagen and HA crystals, causing the release of calcium ions (PMID: 26851678).

SPARC can bind to a number of different ECM proteins, particularly collagen, and may even influence the assembly of these proteins (PMID: 19798598). It is known to regulate interactions between cells and the ECM, meaning it plays a role in many important processes such as cell migration and proliferation.

What diseases are associated with SPARC?

Overexpression of SPARC has been identified in a number of different types of cancers (PMID: 18849185), although its role may vary. For example, in neuroblastomas SPARC acts as a suppressor but in gliomas, it causes greater invasion of the tumor. Mutations in SPARC can also lead to Osteogenesis Imperfecta, also known as brittle bone disease (PMID: 26027498).

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