Anticorps Monoclonal anti-SPARC

SPARC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

1A2C1

N° de cat : 66426-1-Ig

Synonymes

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

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Galerie de données de validation

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  WB analysis of A375 using 66426-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 66426-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66426-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C6 using 66426-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SPARC antibody (66426-1-Ig, Clone: 1A2C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A375, cellule NCCIT, tissu testiculaire humain
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C6,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

66426-1-Ig cible SPARC dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SPARC Protéine recombinante Ag7390
• Nom complet: secreted protein, acidic, cysteine-rich (osteonectin)
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 38 kDa, 52 kDa
• Numéro d’acquisition GenBank: BC004974
• Symbole du gène: SPARC
• Identification du gène (NCBI): 6678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).

Publications

Species	Application	Title
human,mouse	IHC	Adv Sci (Weinh); Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway; Authors - Jiayu Wang
human,mouse	WB	Int J Cancer; Tissue-resident CXCR4+ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression; Authors - Zhenyu Liao
human	WB	Br J Cancer; Inactivation of 3-hydroxybutyrate dehydrogenase type 2 promotes proliferation and metastasis of nasopharyngeal carcinoma by iron retention.; Authors - Bo Li
human	WB	Front Endocrinol (Lausanne); Secreted Protein Acidic and Rich in Cysteine Mediates the Development and Progression of Diabetic Retinopathy.; Authors - Liying Luo
mouse	WB	Exp Cell Res; Sorafenib derivatives-functionalized gold nanoparticles confer protection against tumor angiogenesis and proliferation via suppression of EGFR and VEGFR-2.; Authors - Wei Huang
human	WB	Pathol Res Pract; Downregulation of NEBL promotes migration and invasion of clear cell renal cell carcinoma by inducing epithelial-mesenchymal transition; Authors - Ran Zhao

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