Anticorps Monoclonal anti-SPARC

SPARC Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A2C1

N° de cat : 66426-1-PBS

Synonymes

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

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Galerie de données de validation

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  WB analysis of A375 using 66426-1-Ig (same clone as 66426-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66426-1-Ig (same clone as 66426-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66426-1-Ig (same clone as 66426-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SPARC antibody (66426-1-Ig, Clone: 1A2C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66426-1-PBS cible SPARC dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SPARC Protéine recombinante Ag7390
• Nom complet: secreted protein, acidic, cysteine-rich (osteonectin)
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 38 kDa, 52 kDa
• Numéro d’acquisition GenBank: BC004974
• Symbole du gène: SPARC
• Identification du gène (NCBI): 6678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).

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