Anticorps Recombinant de lapin anti-SPARC

SPARC Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241952E11

N° de cat : 84621-3-PBS

Synonymes

241952E11, Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40

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Galerie de données de validation

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  WB analysis using 84621-3-RR (same clone as 84621-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84621-3-RR (SPARC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84621-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01430-2

  Human milk of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 1,084.0 pg/mL with a range of 425.0 - 1,594.2 pg/mL.

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  Sandwich ELISA sample test of MP01430-2

  Serum of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 212,811.0 pg/mL with a range of 153,761.1 - 333,381.8 pg/mL.

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  Sandwich ELISA sample test of MP01430-2

  MG-63 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for human SPARC, and measured 91,240.8 pg/mL.

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  Sandwich ELISA standard curve of MP01430-2

  Sandwich ELISA standard curve of MP01430-2, Human SPARC Recombinant Matched Antibody Pair - PBS only. 84621-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1169. 84621-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL

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  IHC staining of human stomach cancer using 84621-3-RR (same clone as 84621-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84621-3-RR (SPARC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84621-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84621-3-RR (same clone as 84621-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84621-3-RR (SPARC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84621-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01430-2

  Cytometric bead array standard curve of MP01430-2, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-3-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

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  Affinity and Kinetic Characterization of 84621-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84621-3-RR against Human SPARC were performed. The affinity constant is 1.55 nM.

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Informations sur le produit

84621-3-PBS cible SPARC dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SPARC Protéine recombinante Eg1169
• Nom complet: secreted protein, acidic, cysteine-rich (osteonectin)
• Masse moléculaire calculée: 35kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: NM_003118.4
• Symbole du gène: SPARC
• Identification du gène (NCBI): 6678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).

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