Anticorps Recombinant de lapin anti-SPARC

SPARC Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241952E11

N° de cat : 84621-3-RR

Synonymes

241952E11, Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40

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Galerie de données de validation

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  WB analysis using 84621-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84621-3-RR (SPARC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 84621-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84621-3-RR (SPARC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 84621-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84621-3-RR (SPARC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84621-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84621-3-RR against Human SPARC were performed. The affinity constant is 1.55 nM.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules A375, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84621-3-RR cible SPARC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SPARC Protéine recombinante Eg1169
• Nom complet: secreted protein, acidic, cysteine-rich (osteonectin)
• Masse moléculaire calculée: 35kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: NM_003118.4
• Symbole du gène: SPARC
• Identification du gène (NCBI): 6678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).

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