Anticorps Polyclonal de lapin anti-SPAST

• Phare
• Validé par KD/KO

SPAST Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 22792-1-AP

Synonymes

ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4

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Galerie de données de validation

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  WB analysis using 22792-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22792-1-AP (SPAST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 22792-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPAST antibody (22792-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

22792-1-AP cible SPAST dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPAST Protéine recombinante Ag18797
• Nom complet: spastin
• Masse moléculaire calculée: 616 aa, 67 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC150260
• Symbole du gène: SPAST
• Identification du gène (NCBI): 6683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the growth and regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.

Publications

Species	Application	Title
human	WB	Cell Rep; Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.; Authors - Inna Goliand ; KO Validated
rat	WB	FASEB J; Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.; Authors - Ying Gao
human,mouse	WB,IF	Acta Neuropathol Commun; The enhanced association between mutant CHMP2B and spastin is a novel pathological link between frontotemporal dementia and hereditary spastic paraplegias; Authors - Yongping Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mhd Aghyad (Verified Customer) (09-15-2022)

I used the antibody for western blotting at 1:1000 dilution and it worked well. Clean clear bands could be observed at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK cells