Anticorps Monoclonal anti-SPAST

SPAST Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F5A7

N° de cat : 67361-1-PBS

Synonymes

ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4

---

Galerie de données de validation

• 

  WB analysis using 67361-1-Ig (same clone as 67361-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67361-1-Ig (SPAST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SPAST antibody (67361-1-Ig, Clone: 1F5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67361-1-PBS cible SPAST dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SPAST Protéine recombinante Ag18866
• Nom complet: spastin
• Masse moléculaire calculée: 616 aa, 67 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC150260
• Symbole du gène: SPAST
• Identification du gène (NCBI): 6683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.

{{ptg:RelatedPrimaryAntibodies}}