Anticorps Polyclonal de lapin anti-SPATA22

• Phare
• Validé par KD/KO

SPATA22 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (2)

N° de cat : 16989-1-AP

Synonymes

NYD SP20, SPATA22, spermatogenesis associated 22

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Galerie de données de validation

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  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16989-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16989-1-AP

  IP Result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug.

• 

  IHC staining of mouse testis using 16989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 8 publications below

Informations sur le produit

16989-1-AP cible SPATA22 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPATA22 Protéine recombinante Ag10591
• Nom complet: spermatogenesis associated 22
• Masse moléculaire calculée: 363 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC029483
• Symbole du gène: SPATA22
• Identification du gène (NCBI): 84690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis; Authors - Chao Liu
mouse	IF	Nat Commun; Nutrient restriction synergizes with retinoic acid to induce mammalian meiotic initiation in vitro.; Authors - Xiaoyu Zhang
mouse	WB	Nat Commun; The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.; Authors - Jingjing Zhang
mouse	IF	Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
mouse	IF	Cell Death Differ; A truncating variant of RAD51B associated with primary ovarian insufficiency provides insights into its meiotic and somatic functions.; Authors - Monica M Franca
mouse	WB	Nat Commun; MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.; Authors - Mengcheng Luo ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Murali (Verified Customer) (03-11-2021)

Works nicely.Used on HeLa cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: HeLa cells

SPATA22 Antibody Immunofluorescence validation (1:25 dilution) in HeLa cells (Cat no:16989-1-AP)

FH

Xiaoyu (Verified Customer) (11-04-2019)

good for immunofluorescence

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: germ cell