Anticorps Polyclonal de lapin anti-SPATA22

• Phare
• Validé par KD/KO

SPATA22 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 16989-1-PBS

Synonymes

NYD SP20, SPATA 22, Spermatogenesis-associated protein 22, Testis development protein NYD-SP20

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Galerie de données de validation

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  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16989-1-AP (same clone as 16989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 16989-1-AP (same clone as 16989-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  IP result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16989-1-PBS cible SPATA22 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPATA22 Protéine recombinante Ag10591
• Nom complet: spermatogenesis associated 22
• Masse moléculaire calculée: 363 aa, 41 kDa
• Poids moléculaire observé: 44, 41 kDa
• Numéro d’acquisition GenBank: BC029483
• Symbole du gène: SPATA22
• Identification du gène (NCBI): 84690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.

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