Anticorps Polyclonal de lapin anti-SPATS2

SPATS2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32517-1-PBS

Synonymes

Nbla00526, p59scr, SCR59, Serine-rich spermatocytes and round spermatid 59 kDa protein, SPATA10

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Galerie de données de validation

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  WB analysis using 32517-1-AP (same clone as 32517-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 32517-1-AP (SPATS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32517-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 32517-1-AP (same clone as 32517-1-PBS)

  IP result of anti-SPATS2 (IP:32517-1-AP, 4ug; Detection:32517-1-AP 1:5000) with HeLa cells lysate 1400 ug. This data was developed using the same antibody clone with 32517-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 32517-1-AP (same clone as 32517-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SPATS2 antibody (32517-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 32517-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32517-1-PBS cible SPATS2 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPATS2 Protéine recombinante Ag38507
• Nom complet: spermatogenesis associated, serine-rich 2
• Masse moléculaire calculée: 60kDa，545aa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_001293285.1
• Symbole du gène: SPATS2
• Identification du gène (NCBI): 65244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPATS2, also known as SCR59 and SPATA10, is a member of the RNA-binding proteins whose aberrant expression is associated with carcinogenesis in several types of cancer. It is mainly expressed in the adult testis, with low expression in liver and other tissues (PMID: 33037180). The apparent molecular weight is higher than the calculated molecular weight of 60 kDa, probably due to post-translational modification, probably by phosphorylation.

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