Anticorps Polyclonal de lapin anti-SPATS2L

SPATS2L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29930-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29930-1-PBS cible SPATS2L dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPATS2L Protéine recombinante Ag31157
• Nom complet: viral DNA polymerase-transactivated protein 6
• Masse moléculaire calculée: 488 aa, 54 kDa
• Poids moléculaire observé: 54-65 kDa
• Numéro d’acquisition GenBank: BC018736
• Symbole du gène: SPATS2L
• Identification du gène (NCBI): 26010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

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