Anticorps Polyclonal de lapin anti-SPHK1

• Phare
• Validé par KD/KO

SPHK1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (1)

N° de cat : 10670-1-AP

Synonymes

SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPK, SPK 1

---

Galerie de données de validation

• 

  WB analysis using 10670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10670-1-AP (SPHK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 10670-1-AP

  IP result of anti-SPHK1 (IP:10670-1-AP, 4ug; Detection:10670-1-AP 1:300) with L02 cells lysate 2560 ug.

• 

  IHC staining of human breast cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules L02, cellules MCF-7
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 31 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10670-1-AP cible SPHK1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPHK1 Protéine recombinante Ag1051
• Nom complet: sphingosine kinase 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC008040
• Symbole du gène: SPHK1
• Identification du gène (NCBI): 8877
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

Publications

Species	Application	Title
mouse	WB	Food Funct; The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet; Authors - Kelei Li
human,mouse	WB,IP	Cells; UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1; Authors - Yulia Epshtein
human	WB	Pharmacol Res; Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.; Authors - Jiale Zhu
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
mouse	IF	Int J Mol Sci; Sphingosine Kinases at the Intersection of Pro-Inflammatory LPS and Anti-Inflammatory Endocannabinoid Signaling in BV2 Mouse Microglia Cells; Authors - Sara Standoli
human	WB	Ecotoxicol Environ Saf; Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.; Authors - Liangjing Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Wei (Verified Customer) (03-12-2020)

Strong and specific band at correct size for western blot.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart