Anticorps Polyclonal de lapin anti-SPHK1

• Phare
• Validé par KD/KO

SPHK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(64)

Avis (1)

N° de cat : 10670-1-AP

Synonymes

Acetyltransferase SPHK1, EC:2.3.1.-, EC:2.7.1.91, SK1, Sphingosine kinase 1

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Galerie de données de validation

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  WB analysis using 10670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10670-1-AP (SPHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 10670-1-AP

  IP result of anti-SPHK1 (IP:10670-1-AP, 4ug; Detection:10670-1-AP 1:300) with L02 cells lysate 2560 ug.

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  IHC staining of human breast cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 10670-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using SPHK1 antibody (10670-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 57 publications below
• IHC: See 15 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10670-1-AP cible SPHK1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPHK1 Protéine recombinante Ag1051
• Nom complet: sphingosine kinase 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC008040
• Symbole du gène: SPHK1
• Identification du gène (NCBI): 8877
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

Publications

Species	Application	Title
human	WB	Cell Death Discov; SPHK1 enhances olaparib resistance in ovarian cancer through the NFκB/NRF2/ferroptosis pathway; Authors - Kai Teng
mouse	WB	Food Funct; The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet; Authors - Kelei Li
mouse,human	WB,IP	Cells; UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1; Authors - Yulia Epshtein
human	WB	Pharmacol Res; Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.; Authors - Jiale Zhu
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
mouse,human	WB	Biochem Pharmacol; An inducible sphingosine kinase 1 in hepatic stellate cells potentiates liver fibrosis; Authors - Jin Sol Baek ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Strong and specific band at correct size for western blot.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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