Anticorps Recombinant de lapin anti-SPHK1

SPHK1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2K12

N° de cat : 82609-1-RR

Synonymes

Sphingosine kinase 1, SK1, EC:2.7.1.91, EC:2.3.1.-, Acetyltransferase SPHK1

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Galerie de données de validation

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  WB analysis using 82609-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82609-1-RR (SPHK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82609-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPHK1 antibody (82609-1-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82609-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82609-1-RR against Human SPHK1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82609-1-RR cible SPHK1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SPHK1 Protéine recombinante Ag1051
• Nom complet: sphingosine kinase 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC008040
• Symbole du gène: SPHK1
• Identification du gène (NCBI): 8877
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

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