Anticorps Monoclonal anti-SPI1

SPI1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H3D3

N° de cat : 66618-2-PBS

Synonymes

2H3D3, 31 kDa transforming protein, 31 kDa-transforming protein, OF, PU.1

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Galerie de données de validation

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  WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66618-2-Ig (same clone as 66618-2-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 66618-2-Ig (same clone as 66618-2-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66618-2-Ig (same clone as 66618-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

• 

  IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

• 

  IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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Informations sur le produit

66618-2-PBS cible SPI1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: spleen focus forming virus (SFFV) proviral integration oncogene spi1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 32-38 kDa
• Numéro d’acquisition GenBank: NM_003120
• Symbole du gène: SPI1
• Identification du gène (NCBI): 6688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

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