Anticorps Monoclonal anti-SPIN1

SPIN1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1G8C5

N° de cat : 68615-1-Ig

Synonymes

OCR, Ovarian cancer related protein, SPIN, SPIN1, spindlin 1

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Galerie de données de validation

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  WB analysis using 68615-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68615-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 68615-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules Jurkat, cellules K-562, cellules NCCIT, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68615-1-Ig cible SPIN1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SPIN1 Protéine recombinante Ag33686
• Nom complet: spindlin 1
• Masse moléculaire calculée: 262 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013571
• Symbole du gène: SPIN1
• Identification du gène (NCBI): 10927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

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