Anticorps Monoclonal anti-SPON2

SPON2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H4B11

N° de cat : 66859-1-PBS

Synonymes

1H4B11, Differentially expressed in cancerous and non-cancerous lung cells 1, DIL 1, DIL1, DIL-1

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Galerie de données de validation

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  WB analysis of LNCaP using 66859-1-Ig (same clone as 66859-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66859-1-Ig (SPON2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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  IF Staining of LNCaP using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using SPON2 antibody (66859-1-Ig, Clone: 1H4B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66859-1-PBS cible SPON2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SPON2 Protéine recombinante Ag14337
• Nom complet: spondin 2, extracellular matrix protein
• Masse moléculaire calculée: 331 aa, 36 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC002707
• Symbole du gène: SPON2
• Identification du gène (NCBI): 10417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).

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