Anticorps Polyclonal de lapin anti-SPOP

• Phare
• Validé par KD/KO

SPOP Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

67

N° de cat : 16750-1-AP

Synonymes

HIB homolog 1, Roadkill homolog 1, speckle type POZ protein, SPOP, TEF2

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Galerie de données de validation

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  WB analysis using 16750-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16750-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16750-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16750-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16750-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16750-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16750-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16750-1-AP (SPOP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules PC-3
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 38 publications below
• WB: See 62 publications below
• IHC: See 11 publications below
• IF: See 10 publications below
• IP: See 6 publications below
• CoIP: See 6 publications below
• RIP: See 1 publications below

Informations sur le produit

16750-1-AP cible SPOP dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPOP Protéine recombinante Ag10215
• Nom complet: speckle-type POZ protein
• Masse moléculaire calculée: 374 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC003385
• Symbole du gène: SPOP
• Identification du gène (NCBI): 8405
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The SPOP (TEF2) protein was previously identified as an autoantigen in a patient with scleroderma pigmentosum. SPOP (speckle-type POZ protein), also known as TEF2, HIB homolog 1 or Roadkill homolog 1, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF Homology) domain and one C-terminal BTB/POZ domain. SPOP can exist as a homodimer and is expressed in a variety of tissues localizing to the nucleus. BTB-mediated SPOP dimers form linear oligomers via BACK domain dimerization, and we determine the concentration-dependent populations of the resulting oligomeric species (PMID: 27220849 ). Through an interaction with CUL-3, SPOP is involved in ubiquitinylation and protein degradation. SPOP specifically interacts with CUL-3 via its BTB/POZ domain and recruits substrates to the CUL-3-based ubiquitin ligase via its MATH domain. Substrates recruited by SPOP and targeted for ubiquitylation via the CUL-3/SPOP complex include PDX-1, Bmi-1, MacroH2A, PIPK II ∫ and Daxx. These substrates are subsequently degraded by the proteasome. In addition, SPOP itself becomes ubiquitylated by the CUL-3-based ubiquitin ligase and is targeted for proteasomal degradation.

Publications

Species	Application	Title
human	WB,IP	Nature; Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance.; Authors - Jinfang Zhang ; KO Validated
human	WB	Nat Med; Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.; Authors - Xiangpeng Dai ; KO Validated
human	WB	Nat Med; Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.; Authors - Pingzhao Zhang ; KD Validated
human	WB	Cell Res; ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer.; Authors - Kai Li ; KD Validated
mouse		Mol Cell; DNA Damage Promotes TMPRSS2-ERG Oncoprotein Destruction and Prostate Cancer Suppression via Signaling Converged by GSK3β and WEE1.; Authors - Zhe Hong
human	WB	Mol Cancer; SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.; Authors - Qiwei Jiang ; KD Validated

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