Anticorps Polyclonal de lapin anti-SPR

SPR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 16822-1-AP

Synonymes

EC:1.1.1.153, SDR38C1, Sepiapterin reductase

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Galerie de données de validation

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  WB analysis using 16822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16822-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 16822-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16822-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16822-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16822-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16822-1-AP

  IP result of anti-SPR (IP:16822-1-AP, 4ug; Detection:16822-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver cancer using 16822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16822-1-AP

  Immunofluorescent analysis of Hela cells, using SPR antibody 16822-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562, tissu cardiaque de rat, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

16822-1-AP cible SPR dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPR Protéine recombinante Ag10395
• Nom complet: sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
• Masse moléculaire calculée: 261 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC017310
• Symbole du gène: SPR
• Identification du gène (NCBI): 6697
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.; Authors - Mariluz Soula
mouse	WB	Acta Physiol (Oxf); Tetrahydrobiopterin synthesis and metabolism is impaired in dystrophin-deficient mdx mice and humans.; Authors - Angus Lindsay
human	WB	Phytomedicine; Cimifugin suppresses type 2 airway inflammation by binding to SPR and regulating its protein expression in a non-enzymatic manner; Authors - Xiaoqun Gu
	WB	Cell Death Dis; Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator; Authors - Masamitsu Sone

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