Anticorps Polyclonal de lapin anti-SPRY4

• Phare
• Validé par KD/KO

SPRY4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 22765-1-AP

Synonymes

Spry-4, Spry 4, sprouty homolog 4 (Drosophila), Protein sprouty homolog 4, HH17

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Galerie de données de validation

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  WB analysis of A375 using 22765-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22765-1-AP (SPRY4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 22765-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22765-1-AP (SPRY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 22765-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22765-1-AP (SPRY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A375
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

22765-1-AP cible SPRY4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPRY4 Protéine recombinante Ag18574
• Nom complet: sprouty homolog 4 (Drosophila)
• Masse moléculaire calculée: 299 aa, 33 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC125095
• Symbole du gène: SPRY4
• Identification du gène (NCBI): 81848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPRY4, also named as Protein sprouty homolog 4, is a 299 amino acid protein, which contains one SPR (sprouty) domain and belongs to the sprouty family. SPRY4 Suppresses the INS receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. SPRY4 has a cellular function to regulate the actin cytoskeleton, independent of its inhibitory activity on the Ras/MAP kinase signalling. SPRY1, SPRY2, SPRY3, SPRY4, are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway.

Publications

Species	Application	Title
human	WB	J Clin Invest; Targeting enhancer reprogramming to mitigate MEK inhibitor resistance in preclinical models of advanced ovarian cancer.; Authors - Shini Liu ; KD Validated
human	IHC	Cancer Lett; Coupling HDAC4 with transcriptional factor MEF2D abrogates SPRY4-mediated suppression of ERK activation and elicits hepatocellular carcinoma drug resistance.; Authors - Qingxia Ma ; KO Validated
human	WB,IHC,IF	Am J Reprod Immunol; SPRY4 regulates trophoblast proliferation and apoptosis via regulating IFN-γ-induced STAT1 expression and activation in recurrent miscarriage.; Authors - Shi Qin ; KD Validated
human	WB,IHC	Cell Death Dis; SPRY4 is responsible for pathogenesis of adolescent idiopathic scoliosis by contributing to osteogenic differentiation and melatonin response of bone marrow-derived mesenchymal stem cells.; Authors - Jing Li ; KD Validated
human	WB	Nat Commun; AXL confers intrinsic resistance to osimertinib and advances the emergence of tolerant cells.; Authors - Hirokazu Taniguchi ; KD Validated
human	WB	Adipocyte; SPRY4 promotes adipogenic differentiation of human mesenchymal stem cells through the MEK-ERK1/2 signaling pathway; Authors - Na Li ; KD Validated

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