Anticorps Monoclonal anti-SPTLC1

• Phare
• Validé par KD/KO

SPTLC1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D11D7

N° de cat : 66899-1-PBS

Synonymes

Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50

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Galerie de données de validation

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  WB analysis using 66899-1-Ig (same clone as 66899-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66899-1-Ig (same clone as 66899-1-PBS)

  WB result of SPTLC1 antibody (66899-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66899-1-Ig (same clone as 66899-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPTLC1 antibody (66899-1-Ig, Clone: 2D11D7 ) at dilution of 1:981 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50865-2

  Cytometric bead array standard curve of MP50865-2, SPTLC1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66899-4-PBS. Detection antibody: 66899-1-PBS. Standard:Ag7876. Range: 0.195-100 ng/mL.

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Informations sur le produit

66899-1-PBS cible SPTLC1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SPTLC1 Protéine recombinante Ag7876
• Nom complet: serine palmitoyltransferase, long chain base subunit 1
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC007085
• Symbole du gène: SPTLC1
• Identification du gène (NCBI): 10558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

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