Anticorps Polyclonal de lapin anti-SPZ1

SPZ1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 18931-1-AP

Synonymes

NYD TSP1, spermatogenic leucine zipper 1, SPZ1, Testis specific protein 1, TSP1

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Galerie de données de validation

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  WB analysis of PC-3 using 18931-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 18931-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 18931-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-3 using 18931-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 18931-1-AP (SPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules DU 145, tissu testiculaire humain
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18931-1-AP cible SPZ1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPZ1 Protéine recombinante Ag6995
• Nom complet: spermatogenic leucine zipper 1
• Masse moléculaire calculée: 430 aa, 49 kDa
• Poids moléculaire observé: 49-55 kDa
• Numéro d’acquisition GenBank: BC033798
• Symbole du gène: SPZ1
• Identification du gène (NCBI): 84654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.