Anticorps Monoclonal anti-SQLE

• Phare
• Validé par KD/KO

SQLE Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C9A2

N° de cat : 67206-1-PBS

Synonymes

1C9A2, EC:1.14.14.17, ERG1, squalene epoxidase, Squalene monooxygenase

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Galerie de données de validation

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  WB analysis of A549 using 67206-1-Ig (same clone as 67206-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67206-1-Ig (same clone as 67206-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67206-1-Ig (same clone as 67206-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 67206-1-Ig (same clone as 67206-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SQLE antibody (67206-1-Ig, Clone: 1C9A2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50872-3

  Cytometric bead array standard curve of MP50872-3, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-4-PBS. Standard:Ag3266. Range: 0.195-100 ng/mL.

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  Cytometric bead array standard curve of MP50872-4

  Cytometric bead array standard curve of MP50872-4, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-5-PBS. Standard:Ag3266. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP50872-2

  Cytometric bead array standard curve of MP50872-2, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-3-PBS. Standard:Ag3266. Range: 0.098-100 ng/mL.

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Informations sur le produit

67206-1-PBS cible SQLE dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SQLE Protéine recombinante Ag3266
• Nom complet: squalene epoxidase
• Masse moléculaire calculée: 574 aa, 64 kDa
• Poids moléculaire observé: 50-64 kDa
• Numéro d’acquisition GenBank: BC017033
• Symbole du gène: SQLE
• Identification du gène (NCBI): 6713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)

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