Anticorps Polyclonal de lapin anti-SQRDL

• Phare
• Validé par KD/KO

SQRDL Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17256-1-PBS

Synonymes

CGI 44, CGI-44, EC:1.8.5.8, SQOR, Sulfide dehydrogenase-like

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Galerie de données de validation

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  WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 17256-1-AP (same clone as 17256-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17256-1-AP (same clone as 17256-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17256-1-AP (same clone as 17256-1-PBS)

  IP result of anti-SQRDL (IP:17256-1-AP, 4ug; Detection:17256-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 17256-1-AP (same clone as 17256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17256-1-PBS cible SQRDL dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SQRDL Protéine recombinante Ag10762
• Nom complet: sulfide quinone reductase-like (yeast)
• Masse moléculaire calculée: 450 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC016836
• Symbole du gène: SQRDL
• Identification du gène (NCBI): 58472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

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