Anticorps Monoclonal anti-SQRDL

• Phare
• Validé par KD/KO

SQRDL Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B4E3

N° de cat : 68459-1-PBS

Synonymes

Sulfide dehydrogenase-like, SQOR, EC:1.8.5.8, CGI-44, CGI 44

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Galerie de données de validation

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  WB analysis using 68459-1-Ig (same clone as 68459-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68459-1-Ig (SQRDL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68459-1-Ig (same clone as 68459-1-PBS)

  WB result of SQRDL antibody (68459-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SQRDL transfected A549 cells. This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68459-1-Ig (same clone as 68459-1-PBS)

  IP result of anti-SQRDL (IP:68459-1-Ig, 4ug; Detection:68459-1-Ig 1:3000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 68459-1-Ig (same clone as 68459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68459-1-Ig (SQRDL antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68459-1-Ig (same clone as 68459-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SQRDL antibody (68459-1-Ig, Clone: 1B4E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68459-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68459-1-PBS cible SQRDL dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SQRDL Protéine recombinante Ag10762
• Nom complet: sulfide quinone reductase-like (yeast)
• Masse moléculaire calculée: 450 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC016836
• Symbole du gène: SQRDL
• Identification du gène (NCBI): 58472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

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