Anticorps Polyclonal de lapin anti-SRD5A1

SRD5A1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 26001-1-AP

Synonymes

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

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Galerie de données de validation

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  WB analysis of mouse testis using 26001-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26001-1-AP (SRD5A1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of DU 145 using 26001-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 26001-1-AP (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26001-1-AP (SRD5A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26001-1-AP (SRD5A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules DU 145
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

26001-1-AP cible SRD5A1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRD5A1 Protéine recombinante Ag23351
• Nom complet: steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC007033
• Symbole du gène: SRD5A1
• Identification du gène (NCBI): 6715
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Steroid 5-alpha-reductase (SRD5A1) catalyzes the conversion of 17β-Hydroxyandrost-4-en-3-one into the more potent androgen, DHT. SRD5A1 is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

Publications

Species	Application	Title
rat	WB	Sci Rep; Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5α-Reductase.; Authors - Jiakuan Liu
mouse	WB	J Steroid Biochem Mol Biol; Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.; Authors - Qi Wang
rat	WB	J Integr Med; Rauwolfia vomitoria extract suppresses benign prostatic hyperplasia by reducing expression of androgen receptor and 5α-reductase in a rat model.; Authors - Tian Fang
mouse	IF	Biomedicines; Mixed-Culture Propagation of Uterine-Tissue-Resident Macrophages and Their Expression Properties of Steroidogenic Molecules; Authors - Kazushige Ogawa