Anticorps Monoclonal anti-SRD5A1

• Phare
• Validé par KD/KO

SRD5A1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D5D8

N° de cat : 66329-1-PBS

Synonymes

Steroid 5 alpha reductase 1, SR type 1, S5AR 1, EC:1.3.1.22, 3-oxo-5-alpha-steroid 4-dehydrogenase 1

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Galerie de données de validation

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  WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

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  WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

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  IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using SRD5A1 antibody (66329-1-Ig, Clone: 1D5D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50930-1

  Cytometric bead array standard curve of MP50930-1, SRD5A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66329-1-PBS. Detection antibody: 66329-2-PBS. Standard:Ag23351. Range: 0.781-100 ng/mL.

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Informations sur le produit

66329-1-PBS cible SRD5A1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SRD5A1 Protéine recombinante Ag23351
• Nom complet: steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC007033
• Symbole du gène: SRD5A1
• Identification du gène (NCBI): 6715
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Steroid 5-alpha-reductase (SRD5A1)   is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

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