Anticorps Monoclonal anti-SREBF1

• Phare
• Validé par KD/KO

SREBF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B6G5

N° de cat : 66875-1-PBS

Synonymes

1B6G5, bHLHd1, Class D basic helix-loop-helix protein 1, Processed sterol regulatory element-binding protein 1, SREBF 1

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Galerie de données de validation

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  WB analysis of HeLa using 66875-1-Ig (same clone as 66875-1-PBS)

  WB result of SREBF1 antibody (66875-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin stains the F-actin (red). DAPI (blue). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66875-1-Ig (SREBF1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66875-1-PBS cible SREBF1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SREBF1 Protéine recombinante Ag5484
• Nom complet: sterol regulatory element binding transcription factor 1
• Masse moléculaire calculée: 1177 aa, 125 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC063281
• Symbole du gène: SREBF1
• Identification du gène (NCBI): 6720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.

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