Anticorps Polyclonal de lapin anti-SREBF2

• Phare
• Validé par KD/KO

SREBF2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(67)

Avis (5)

N° de cat : 28212-1-AP

Synonymes

bHLHd2, Class D basic helix-loop-helix protein 2, Processed sterol regulatory element-binding protein 2, SREBF 2, SREBP 2

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Galerie de données de validation

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  WB analysis using 28212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28212-1-AP

  IP result of anti-SREBF2 (IP:28212-1-AP, 4ug; Detection:28212-1-AP 1:1500) with HeLa cells lysate 1200 ug.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules HepG2, cellules PC-3
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 68 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

28212-1-AP cible SREBF2 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SREBF2 Protéine recombinante Ag28205
• Nom complet: sterol regulatory element binding transcription factor 2
• Masse moléculaire calculée: 124 kDa
• Poids moléculaire observé: 73-75 kDa, 120-130 kDa
• Numéro d’acquisition GenBank: BC056158
• Symbole du gène: SREBF2
• Identification du gène (NCBI): 6721
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. SREBF2 exists two isoform with molecular weight 124 kDa and 73 kDa.

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
Human	WB	Cancer Cell; Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis; Authors - Wei Zhou
human	WB	Redox Biol; Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation; Authors - Youzi Kong ; KD Validated
human	WB	Hypertension; Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia; Authors - Lunbo Tan
mouse	WB	Acta Pharmacol Sin; Urolithin A promotes atherosclerotic plaque stability by limiting inflammation and hypercholesteremia in Apolipoprotein E-deficient mice; Authors - Meng-Yun Xu
human	WB	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Simon (Verified Customer) (07-23-2025)

The antibody works well for Jurkat cells, showin the precursor and the mature SREBF2

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: lymphoblasts (Jurkat cell line)

SREBF2 Antibody Western Blot validation (1:4000 dilution) in lymphoblasts (Jurkat cell line) (Cat no:28212-1-AP)

FH

Shunbin (Verified Customer) (05-14-2024)

Not good. Only non specific bands detected

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B cell lines

SREBF2 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:28212-1-AP)

FH

Shunbin (Verified Customer) (05-14-2024)

Many non specific bands, but no specific band can be identified

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

SREBF2 Antibody Western Blot validation (1:1000 dilution) in (Cat no:28212-1-AP)

FH

Bertrand (Verified Customer) (04-08-2024)

This is a Srebp2 antibody, however, the results from western blot analysis show that the product might not be specific to Srebf2...There are lots of non-specific bands and the uncleaved/immature protein should not be present in the nucleus of cells, yet it was there.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver homogenates

FH

Boyan (Verified Customer) (04-17-2023)

OK for WB, a specific but weak band was seen around 150 kd, but not 130 kd, which is shown on its booklet. The 130 kd band is a strong nonspecific band.

• Applications: Western Blot

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