Anticorps Monoclonal anti-SRF

• Phare
• Validé par KD/KO

SRF Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G8F4

N° de cat : 66742-1-PBS

Synonymes

1G8F4, MCM1, Serum response factor

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Galerie de données de validation

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  WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66742-1-Ig (same clone as 66742-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66742-1-Ig (same clone as 66742-1-PBS)

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66742-1-PBS cible SRF dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SRF Protéine recombinante Ag10386
• Nom complet: serum response factor (c-fos serum response element-binding transcription factor)
• Masse moléculaire calculée: 508 aa, 52 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC048211
• Symbole du gène: SRF
• Identification du gène (NCBI): 6722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

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