Anticorps Polyclonal de lapin anti-SRP14

• Phare
• Validé par KD/KO

SRP14 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 11528-1-AP

Synonymes

18 kDa Alu RNA binding protein, ALURBP, SRP14

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Galerie de données de validation

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  WB analysis using 11528-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11528-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11528-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11528-1-AP

  IP Result of anti-SRP14 (IP:11528-1-AP, 4ug; Detection:11528-1-AP 1:300) with A549 cells lysate 1600ug.

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  IF Staining of MCF-7 using 11528-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SRP14 antibody 11528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 11528-1-AP

  Immunofluorescent analysis of HepG2 cells, using SRP14 antibody 11528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, tissu cardiaque humain
• Résultats positifs en IP: cellules A549
• Résultats positifs en IF: cellules MCF-7, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IP: See 1 publications below

Informations sur le produit

11528-1-AP cible SRP14 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRP14 Protéine recombinante Ag2099
• Nom complet: signal recognition particle 14kDa (homologous Alu RNA binding protein)
• Masse moléculaire calculée: 136 aa, 15 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC035495
• Symbole du gène: SRP14
• Identification du gène (NCBI): 6727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal recognition particle(SRP) acts in three distinct ways: 1 it binds to the signal sequence of the nascent polypeptide to be translocated, which is exposed on the surface of the translating ribosome; 2 it temporarily retards the nascent polypeptide from further elongation; 3 it mediates docking of the SRP-ribosome-nascent polypeptide chain complex to the RER membrane via the heterodimeric SRP-receptor (SR). SRP14, also named as 18 kDa Alu RNA-binding protein, is a 136 amino acid protein. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. SRP14 protein is a component of signal-recognition-particle that has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. SRP14 form a heterodimer with SRP9, which recognizes Alu RNA and the related 7SL RNA.

Publications

Species	Application	Title
human	WB	Cell; Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.; Authors - Barzin Y Nabet ; KD Validated
human	WB	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	Genes Dev; Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs.; Authors - Jády Beáta E BE
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
human	WB	Nucleic Acids Res; Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.; Authors - Piazzon Nathalie N
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy