Anticorps Monoclonal anti-SRP54

SRP54 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D6D1

N° de cat : 67005-1-PBS

Synonymes

1D6D1, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54

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Galerie de données de validation

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  WB analysis using 67005-1-Ig (same clone as 67005-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67005-1-Ig (SRP54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67005-1-Ig (same clone as 67005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67005-1-Ig (SRP54 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67005-1-Ig (same clone as 67005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67005-1-Ig (SRP54 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67005-1-Ig (same clone as 67005-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (67005-1-Ig, Clone: 1D6D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67005-1-Ig (same clone as 67005-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SRP54 (67005-1-Ig, Clone:1D6D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67005-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67005-1-PBS cible SRP54 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SRP54 Protéine recombinante Ag12166
• Nom complet: signal recognition particle 54kDa
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC003389
• Symbole du gène: SRP54
• Identification du gène (NCBI): 6729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

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