Anticorps Recombinant de lapin anti-SRP54

SRP54 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241125G1

N° de cat : 84014-6-PBS

Synonymes

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

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Galerie de données de validation

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  WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

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  WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84014-6-RR (same clone as 84014-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84014-6-RR (same clone as 84014-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (84014-6-RR, Clone: 241125G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84014-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

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Informations sur le produit

84014-6-PBS cible SRP54 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SRP54 Protéine recombinante Ag2327
• Nom complet: signal recognition particle 54kDa
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC003389
• Symbole du gène: SRP54
• Identification du gène (NCBI): 6729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

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