Anticorps Monoclonal anti-SRP9

• Phare
• Validé par KD/KO

SRP9 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1H7G10

N° de cat : 66068-1-Ig

Synonymes

ALURBP, SRP9

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Galerie de données de validation

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  WB analysis using 66068-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66068-1-Ig

  WB result of SRP9 antibody (66068-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP9 transfected HeLa cells.

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  WB analysis of HeLa using 66068-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66068-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66068-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66068-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules 4T1, cellules HEK-293, cellules HeLa, cellules NIH/3T3, cellules ROS1728

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66068-1-Ig cible SRP9 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SRP9 Protéine recombinante Ag17153
• Nom complet: signal recognition particle 9kDa
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC015094
• Symbole du gène: SRP9
• Identification du gène (NCBI): 6726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.

Publications

Species	Application	Title
human	WB	bioRxiv; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan
human	WB	Nucleic Acids Res; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan

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