Anticorps Recombinant de lapin anti-SRPK2

SRPK2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242935B1

N° de cat : 85613-5-RR

Synonymes

EC:2.7.11.1, Serine/arginine-rich protein-specific kinase 2, SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2

---

Galerie de données de validation

• 

  WB analysis using 85613-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85613-5-RR (SRPK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 85613-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85613-5-RR (SRPK2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85613-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85613-5-RR against Human SRPK2 were performed. The affinity constant is 4.16 nM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85613-5-RR cible SRPK2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SRPK2 Protéine recombinante Ag17885
• Nom complet: SFRS protein kinase 2
• Masse moléculaire calculée: 688 aa, 78 kDa
• Poids moléculaire observé: 105-110 kDa
• Numéro d’acquisition GenBank: BC035214
• Symbole du gène: SRPK2
• Identification du gène (NCBI): 6733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

{{ptg:RelatedPrimaryAntibodies}}