Anticorps Polyclonal de lapin anti-SRRM1

• Phare
• Validé par KD/KO

SRRM1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12822-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse liver using 12822-1-AP (same clone as 12822-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

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  WB analysis using 12822-1-AP (same clone as 12822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12822-1-AP (same clone as 12822-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

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  WB analysis of UV treated HEK-293 using 12822-1-AP (same clone as 12822-1-PBS)

  UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

  IP result of anti-SRRM1 (IP:12822-1-AP, 4ug; Detection:12822-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12822-1-PBS cible SRRM1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SRRM1 Protéine recombinante Ag3513
• Nom complet: serine/arginine repetitive matrix 1
• Masse moléculaire calculée: 904 aa, 102 kDa
• Poids moléculaire observé: 120-160 kDa
• Numéro d’acquisition GenBank: BC036187
• Symbole du gène: SRRM1
• Identification du gène (NCBI): 10250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.

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